BAIT

AGTR1

AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, HAT1R
angiotensin II receptor, type 1
GO Process (27)
GO Function (5)
GO Component (3)
Homo sapiens
PREY

ARRB1

ARB1, ARR1
arrestin, beta 1
GO Process (22)
GO Function (9)
GO Component (11)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation.

Barnes WG, Reiter E, Violin JD, Ren XR, Milligan G, Lefkowitz RJ

beta-Arrestins were initially shown, in conjunction with G protein-coupled receptor kinases, to be involved in the desensitization and internalization of activated seven-transmembrane receptors. Recently, beta-arrestin 2 has been shown to act as a signal mediator in mitogen-activated protein kinase cascades and to play a positive regulatory role in chemotaxis. We now show that beta-arrestin 1 is required to activate the ... [more]

J. Biol. Chem. Mar. 04, 2005; 280(9);8041-50 [Pubmed: 15611106]

Throughput

  • Low Throughput

Additional Notes

  • cells stimulated with 100 nM angiotensin II (Ang II) or 10 microM Ang II analog SII

Curated By

  • BioGRID