BAIT

TBXA2R

BDPLT13, TXA2-R
thromboxane A2 receptor
Arachidonic Acid Pathway Project
Homo sapiens
PREY

PRDX4

AOE37-2, AOE372, HEL-S-97n, PRX-4
peroxiredoxin 4
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Peroxiredoxin-4 interacts with and regulates the thromboxane A(2) receptor.

Giguere P, Turcotte ME, Hamelin E, Parent A, Brisson J, Laroche G, Labrecque P, Dupuis G, Parent JL

We identified peroxiredoxin-4 (Prx-4) as a protein interacting with the beta isoform of the thromboxane A(2) receptor (TPbeta) by yeast two-hybrid analysis. Prx-4 co-immunoprecipitated constitutively with TPbeta in HEK293 cells. The second and third intracellular loops as well as the C-terminus of TPbeta interacted directly with Prx-4. Co-expression of Prx-4 caused a 60% decrease in cell surface expression of TPbeta. ... [more]

FEBS Lett. Aug. 07, 2007; 581(20);3863-8 [Pubmed: 17644091]

Throughput

  • Low Throughput

Additional Notes

  • Addition of TBXA2R agonist did not change the interaction
  • Isoforms (Uniprot IDs):P21731-2 interacts with Q13162
  • Oxidative stress induced PRDX4-dependent degradation of the receptor

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TBXA2R PRDX4
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
TBXA2R PRDX4
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
904086

Curated By

  • BioGRID