BAIT

PTGER3

EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3e, PGE2-R, RP5-952N6.2
prostaglandin E receptor 3 (subtype EP3)
Arachidonic Acid Pathway Project
Homo sapiens
PREY

MKLN1

TWA2
muskelin 1, intracellular mediator containing kelch motifs
UPS Project
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Receptor isoform-specific interaction of prostaglandin EP3 receptor with muskelin.

Hasegawa H, Katoh H, Fujita H, Mori K, Negishi M

By using the yeast two-hybrid system, muskelin was found to bind with the carboxy-terminal tail of the prostaglandin EP3 receptor alpha isoform but not with either the beta or gamma isoform. A direct interaction between the carboxy-terminal tail of the alpha isoform and muskelin was confirmed in vitro using recombinant fusion proteins. Analysis by confocal microscopy indicated that the isoform ... [more]

Biochem. Biophys. Res. Commun. Sep. 16, 2000; 276(1);350-4 [Pubmed: 11006128]

Throughput

  • Low Throughput

Additional Notes

  • EP3 alpha C-terminal tail interacted with muskelin
  • Isoforms (Uniprot IDs): P43115-2 interacts with Q9UL63

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PTGER3 MKLN1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
904157

Curated By

  • BioGRID