Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Receptor isoform-specific interaction of prostaglandin EP3 receptor with muskelin.

Hasegawa H, Katoh H, Fujita H, Mori K, Negishi M

By using the yeast two-hybrid system, muskelin was found to bind with the carboxy-terminal tail of the prostaglandin EP3 receptor alpha isoform but not with either the beta or gamma isoform. A direct interaction between the carboxy-terminal tail of the alpha isoform and muskelin was confirmed in vitro using recombinant fusion proteins. Analysis by confocal microscopy indicated that the isoform ... [more]

Biochem. Biophys. Res. Commun. Sep. 16, 2000; 276(1);350-4 [Pubmed: 11006128]

Throughput

Low Throughput

Additional Notes

EP3 alpha C-terminal tail interacted with muskelin
Isoforms (Uniprot IDs): P43115-2 interacts with Q9UL63

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PTGER3 MKLN1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hasegawa H (2000)	Low	-	BioGRID	904157

Curated By

BioGRID

Interaction Summary

PTGER3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [TAS]prostaglandin E receptor activity [NAS]

Gene Ontology Cellular Component

MKLN1