BAIT

BCK1

LAS3, SAP3, SLK1, SSP31, mitogen-activated protein kinase kinase kinase BCK1, L000000162, YJL095W

MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase

Kinome Project (Sc)

GO Process (8)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

endoplasmic reticulum unfolded protein response [IMP]

establishment of cell polarity [IMP]

intracellular signal transduction [IMP]

peroxisome degradation [IMP]

protein phosphorylation [IDA, TAS]

regulation of fungal-type cell wall organization [IGI, IMP]

response to acidic pH [IMP]

response to nutrient [IMP]

Gene Ontology Molecular Function

MAP kinase kinase kinase activity [IGI]
protein kinase activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

mating projection tip [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SWI4

ART1, SBF complex DNA-binding subunit SWI4, L000000124, L000002252, YER111C

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]

positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

SBF transcription complex [IDA, IMP, IPI]

nuclear chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.

Sharifpoor S, van Dyk D, Costanzo M, Baryshnikova A, Friesen H, Douglas AC, Youn JY, Vandersluis B, Myers CL, Papp B, Boone C, Andrews BJ

A combinatorial genetic perturbation strategy was applied to interrogate the yeast kinome on a genome-wide scale. We assessed the global effects of gene overexpression or gene deletion to map an integrated genetic interaction network of synthetic dosage lethal (SDL) and loss-of-function genetic interactions (GIs) for 92 kinases, producing a meta-network of 8700 GIs enriched for pathways known to be regulated ... [more]

Unknown Feb. 17, 2012; 0(0); [Pubmed: 22282571]

Quantitative Score

-0.209 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: colony size (APO:0000063)

Additional Notes

score threshold <= -0.12, interaction detected by Synthetic Genetic Array (SGA)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BCK1 SWI4	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Madden K (1997)	Low	-	BioGRID	429259
BCK1 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Bandyopadhyay S (2010)	High	-13.0466	BioGRID	541717
SWI4 BCK1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.209	BioGRID	375850
BCK1 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.209	BioGRID	390607
SWI4 BCK1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2222	BioGRID	2109548
BCK1 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1937	BioGRID	2135952
SWI4 BCK1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Fiedler D (2009)	High	-14.9796	BioGRID	323994
SWI4 BCK1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Corcoles-Saez I (2012)	Low	-	BioGRID	669497

Curated By

BioGRID

Interaction Summary

BCK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

MAP kinase kinase kinase activity [IGI]protein kinase activity [IDA]

Gene Ontology Cellular Component

SWI4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

MAP kinase kinase kinase activity [IGI]
protein kinase activity [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]