BAIT
EEF1D
EF-1D, EF1D, FP1047, UNQ601
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
GO Process (6)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
ZW10
HZW10, KNTC1AP
zw10 kinetochore protein
GO Process (11)
GO Function (2)
GO Component (8)
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IMP]
- Golgi organization [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- meiotic nuclear division [TAS]
- mitotic cell cycle [TAS]
- mitotic cell cycle checkpoint [IDA]
- mitotic metaphase plate congression [IMP]
- mitotic sister chromatid segregation [IDA]
- protein complex assembly [IDA]
- protein localization to kinetochore [IMP]
- regulation of exit from mitosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]
Throughput
- High Throughput
Ontology Terms
- hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Curated By
- BioGRID