BAIT

CSNK2A2

CK2A2, CSNK2A1
casein kinase 2, alpha prime polypeptide
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CDC5L

CDC5, CDC5-LIKE, CEF1, PCDC5RP, dJ319D22.1, RP1-319D22.1
cell division cycle 5-like
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.

Varjosalo M, Sacco R, Stukalov A, van Drogen A, Planyavsky M, Hauri S, Aebersold R, Bennett KL, Colinge J, Gstaiger M, Superti-Furga G

The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]

Nat. Methods Apr. 01, 2013; 10(4);307-14 [Pubmed: 23455922]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]

Additional Notes

  • exogenous expression of bait

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CSNK2A2 CDC5L
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High918BioGRID
3481587
CSNK2A2 CDC5L
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.92BioGRID
1189636
CSNK2A2 CDC5L
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.7757BioGRID
2270744

Curated By

  • BioGRID