PRKACB
Gene Ontology Biological Process
- activation of phospholipase C activity [TAS]
- activation of protein kinase A activity [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]
- blood coagulation [TAS]
- carbohydrate metabolic process [TAS]
- cellular response to glucagon stimulus [TAS]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- protein phosphorylation [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- transmembrane transport [TAS]
- triglyceride catabolic process [TAS]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRKAR1A
Gene Ontology Biological Process
- activation of phospholipase C activity [TAS]
- activation of protein kinase A activity [TAS]
- blood coagulation [TAS]
- cellular response to glucagon stimulus [TAS]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- negative regulation of cAMP-dependent protein kinase activity [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of insulin secretion [TAS]
- regulation of transcription from RNA polymerase II promoter [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]
Additional Notes
- exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKACB PRKAR1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 31756 | BioGRID | 3481699 | |
| PRKACB PRKAR1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9464 | BioGRID | 3550374 | |
| PRKAR1A PRKACB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 1190080 | |
| PRKACB PRKAR1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2217454 | |
| PRKACB PRKAR1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3130453 | |
| PRKAR1A PRKACB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| PRKACB PRKAR1A | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3791631 |
Curated By
- BioGRID