ATG16L1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ULK1
Gene Ontology Biological Process
- autophagic vacuole assembly [IBA]
- axon extension [IBA]
- cellular response to nutrient levels [ISS]
- neuron projection development [IMP]
- positive regulation of autophagy [ISS]
- positive regulation of macroautophagy [IMP]
- protein autophosphorylation [IDA]
- protein localization [IMP]
- protein phosphorylation [NAS]
- regulation of autophagy [IDA, IMP]
- response to starvation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ATG1/UKL1 signaling complex [IBA]
- ULK1-ATG13-FIP200 complex [IPI]
- autophagic vacuole [IDA]
- cytoplasm [NAS]
- cytosol [IDA]
- extrinsic component of autophagic vacuole membrane [IDA]
- extrinsic component of omegasome membrane [IDA]
- extrinsic component of pre-autophagosomal structure membrane [IDA]
- pre-autophagosomal structure membrane [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy.
Autophagy is a finely orchestrated cellular catabolic process that requires multiple autophagy-related gene products (ATG proteins). The ULK1 complex functions to integrate upstream signals to downstream ATG proteins through an unknown mechanism. Here we have identified an interaction between mammalian FIP200 and ATG16L1, essential components of the ULK1 and ATG5 complexes, respectively. Further analyses show this is a direct interaction ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATG16L1 ULK1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| ATG16L1 ULK1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ATG16L1 ULK1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID