BRF
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TRF
Gene Ontology Biological Process
- embryo development [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription from RNA polymerase III promoter [IDA, TAS]
- transcription from RNA polymerase II promoter [ISS]
- transcription from RNA polymerase III promoter [IDA]
- transcription initiation from RNA polymerase II promoter [IDA, ISS]
- transcription initiation from RNA polymerase III promoter [IEP]
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IDA]
- TFIIA-class transcription factor binding [IDA]
- TFIIB-class transcription factor binding [IPI]
- core promoter sequence-specific DNA binding [IPI]
- transcription factor binding [IPI]
- DNA binding [IDA, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IDA]
- TFIIA-class transcription factor binding [IDA]
- TFIIB-class transcription factor binding [IPI]
- core promoter sequence-specific DNA binding [IPI]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A TRF1:BRF complex directs Drosophila RNA polymerase III transcription.
It has been generally accepted that the TATA binding protein (TBP) is a universal mediator of transcription by RNA polymerase I, II, and III. Here we report that the TBP-related factor TRF1 rather than TBP is responsible for RNA polymerase III transcription in Drosophila. Immunoprecipitation and in vitro transcription assays using immunodepleted extracts supplemented with recombinant proteins reveals that a ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRF BRF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| TRF BRF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 33.5145 | BioGRID | 2866551 | |
| TRF BRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID