TRF
Gene Ontology Biological Process
- embryo development [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription from RNA polymerase III promoter [IDA, TAS]
- transcription from RNA polymerase II promoter [ISS]
- transcription from RNA polymerase III promoter [IDA]
- transcription initiation from RNA polymerase II promoter [IDA, ISS]
- transcription initiation from RNA polymerase III promoter [IEP]
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IDA]
- TFIIA-class transcription factor binding [IDA]
- TFIIB-class transcription factor binding [IPI]
- core promoter sequence-specific DNA binding [IPI]
- transcription factor binding [IPI]
- DNA binding [IDA, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IDA]
- TFIIA-class transcription factor binding [IDA]
- TFIIB-class transcription factor binding [IPI]
- core promoter sequence-specific DNA binding [IPI]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
BRF
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A protein complex network of Drosophila melanogaster.
Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRF BRF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 33.5145 | BioGRID | 2866551 | |
| BRF TRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TRF BRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID