TRF
Gene Ontology Biological Process
- embryo development [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription from RNA polymerase III promoter [IDA, TAS]
- transcription from RNA polymerase II promoter [ISS]
- transcription from RNA polymerase III promoter [IDA]
- transcription initiation from RNA polymerase II promoter [IDA, ISS]
- transcription initiation from RNA polymerase III promoter [IEP]
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IDA]
- TFIIA-class transcription factor binding [IDA]
- TFIIB-class transcription factor binding [IPI]
- core promoter sequence-specific DNA binding [IPI]
- transcription factor binding [IPI]
- DNA binding [IDA, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IDA]
- TFIIA-class transcription factor binding [IDA]
- TFIIB-class transcription factor binding [IPI]
- core promoter sequence-specific DNA binding [IPI]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
BRF
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transcription factor networks in Drosophila melanogaster.
Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately ... [more]
Quantitative Score
- 33.514508 [HGSCore]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified using a HyperGeometric Spectral Counts scoring method (HGSCore) and were filtered to a false discovery rate (FDR) of 2%.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRF BRF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| BRF TRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TRF BRF | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID