BAIT

PBS2

orf6.8548, CaO6.8548, CA5686, IPF3329.1, orf19.7388, C3_06070C, C3_06070C_B, CaO19.7388, IPF3329, Contig4-3036_0020, CAWG_02916, C3_06070C_A
MAPK kinase (MAPKK); role in osmotic and oxidative stress responses, oxidative stress adaptation; required for stress regulation of Hog1p localization and activity; functional homolog of S. cerevisiae Pbs2p
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)
PREY

HOG1

IPF26431.1, CaO19.895, CaO6.7282, orf19.895, CaO19.8514, CAWG_04100, C2_03330C, C2_03330C_B, Contig4-3099_0012, CA4677, orf6.7282, IPF3669.2, orf19.8514, C2_03330C_A
MAP kinase of osmotic-, heavy metal-, and core stress response; role in regulation of glycerol, D-arabitol in response to stress; phosphorylated in response to H2O2 (Ssk1-dependent) or NaCl; mutant induces protective mouse immune response
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Detection of protein-protein interactions through vesicle targeting.

Boysen JH, Fanning S, Newberg J, Murphy RF, Mitchell AP

The detection of protein-protein interactions through two-hybrid assays has revolutionized our understanding of biology. The remarkable impact of two-hybrid assay platforms derives from their speed, simplicity, and broad applicability. Yet for many organisms, the need to express test proteins in Saccharomyces cerevisiae or Escherichia coli presents a substantial barrier because variations in codon specificity or bias may result in aberrant ... [more]

Genetics May. 01, 2009; 182(1);33-9 [Pubmed: 19307607]

Throughput

  • Low Throughput

Additional Notes

  • vesicle capture interaction assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PBS2 HOG1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PBS2 HOG1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
HOG1 PBS2
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
2450520

Curated By

  • BioGRID