BAIT

PBS2

orf6.8548, CaO6.8548, CA5686, IPF3329.1, orf19.7388, C3_06070C, C3_06070C_B, CaO19.7388, IPF3329, Contig4-3036_0020, CAWG_02916, C3_06070C_A

MAPK kinase (MAPKK); role in osmotic and oxidative stress responses, oxidative stress adaptation; required for stress regulation of Hog1p localization and activity; functional homolog of S. cerevisiae Pbs2p

GO Process (0)

GO Function (0)

GO Component (0)

Entrez Gene RefSeq UniprotKB CGD

Candida albicans (SC5314)

PREY

HOG1

IPF26431.1, CaO19.895, CaO6.7282, orf19.895, CaO19.8514, CAWG_04100, C2_03330C, C2_03330C_B, Contig4-3099_0012, CA4677, orf6.7282, IPF3669.2, orf19.8514, C2_03330C_A

MAP kinase of osmotic-, heavy metal-, and core stress response; role in regulation of glycerol, D-arabitol in response to stress; phosphorylated in response to H2O2 (Ssk1-dependent) or NaCl; mutant induces protective mouse immune response

GO Process (0)

GO Function (0)

GO Component (0)

Entrez Gene RefSeq UniprotKB CGD

Candida albicans (SC5314)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MAPKKK-independent regulation of the Hog1 stress-activated protein kinase in Candida albicans.

Cheetham J, MacCallum DM, Doris KS, da Silva Dantas A, Scorfield S, Odds F, Smith DA, Quinn J

The Hog1 stress-activated protein kinase regulates both stress responses and morphogenesis in Candida albicans and is essential for the virulence of this major human pathogen. Stress-induced Hog1 phosphorylation is regulated by the upstream MAPKK, Pbs2, which in turn is regulated by the MAPKKK, Ssk2. Here, we have investigated the role of phosphorylation of Hog1 and Pbs2 in Hog1-mediated processes in ... [more]

J. Biol. Chem. Dec. 09, 2011; 286(49);42002-16 [Pubmed: 21994942]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PBS2 HOG1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cheetham J (2007)	Low	-	BioGRID	-
HOG1 PBS2	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Argimon S (2011)	Low	-	BioGRID	2450520
PBS2 HOG1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Boysen JH (2009)	Low	-	BioGRID	2347363

Curated By

BioGRID

Interaction Summary