BAIT

HOG1

IPF26431.1, CaO19.895, CaO6.7282, orf19.895, CaO19.8514, CAWG_04100, C2_03330C, C2_03330C_B, Contig4-3099_0012, CA4677, orf6.7282, IPF3669.2, orf19.8514, C2_03330C_A

MAP kinase of osmotic-, heavy metal-, and core stress response; role in regulation of glycerol, D-arabitol in response to stress; phosphorylated in response to H2O2 (Ssk1-dependent) or NaCl; mutant induces protective mouse immune response

GO Process (0)

GO Function (0)

GO Component (0)

Entrez Gene RefSeq UniprotKB CGD

Candida albicans (SC5314)

PREY

PBS2

orf6.8548, CaO6.8548, CA5686, IPF3329.1, orf19.7388, C3_06070C, C3_06070C_B, CaO19.7388, IPF3329, Contig4-3036_0020, CAWG_02916, C3_06070C_A

MAPK kinase (MAPKK); role in osmotic and oxidative stress responses, oxidative stress adaptation; required for stress regulation of Hog1p localization and activity; functional homolog of S. cerevisiae Pbs2p

GO Process (0)

GO Function (0)

GO Component (0)

Entrez Gene RefSeq UniprotKB CGD

Candida albicans (SC5314)

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Interaction between the Candida albicans high-osmolarity glycerol (HOG) pathway and the response to human beta-defensins 2 and 3.

Argimon S, Fanning S, Blankenship JR, Mitchell AP

Human Î²-defensins 2 and 3 are small cationic peptides with antimicrobial activity against the fungal pathogen Candida albicans. We found that hog1 and pbs2 mutants were hypersensitive to treatment with these peptides, pointing to a role of the high-osmolarity glycerol (HOG) pathway in the response to defensin-induced cell injury. ... [more]

Eukaryotic Cell Feb. 01, 2011; 10(2);272-5 [Pubmed: 21131433]

Throughput

Low Throughput

Additional Notes

Vesicle-capture interaction (VCI) assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PBS2 HOG1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cheetham J (2007)	Low	-	BioGRID	-
PBS2 HOG1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cheetham J (2011)	Low	-	BioGRID	-
PBS2 HOG1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Boysen JH (2009)	Low	-	BioGRID	2347363

Curated By

BioGRID

Interaction Summary