BAIT

SGS1

ATP-dependent DNA helicase SGS1, L000001877, YMR190C
RecQ family nucleolar DNA helicase; role in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; stimulates DNA catenation/decatenation activity of Top3p; potential repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

CDC48

AAA family ATPase CDC48, L000000280, YDL126C
AAA ATPase; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing, in macroautophagy, PMN, RAD, ribophagy, homotypic ER membrane fusion, disassembly of Met30p from SCF complex; functional ortholog of human p97/VCP
GO Process (18)
GO Function (3)
GO Component (11)
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.

Sanders E, Nguyen PA, Rogers CM, Bochman ML

Most eukaryotic genomes encode multiple RecQ family helicases, including five such enzymes in humans. For many years, the yeast Saccharomyces cerevisiae was considered unusual in that it only contained a single RecQ helicase, named Sgs1 However, it has recently been discovered that a second RecQ helicase, called Hrq1, resides in yeast. Both Hrq1 and Sgs1 are involved in genome integrity, ... [more]

G3 (Bethesda) Dec. 03, 2020; 10(12);4359-4368 [Pubmed: 33115720]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • SGA with hrq1-K318A sgs1-K706A double mutant as query against temperature-sensitive collection, see Table S17 for ts allele (hit); genetic complex
  • SGA with hrq1-K318A sgs1-deletion double mutant as query against temperature-sensitive collection, see Table S16 for ts allele (hit); genetic complex
  • SGA with hrq1-deletion sgs1-K706A double mutant as query against temperature-sensitive collection, see Table S15 for ts allele (hit); genetic complex
  • SGA with hrq1-deletion sgs1-deletion double mutant as query against temperature-sensitive collection, see Table S14 for ts allele (hit); genetic complex
  • SGA with sgs1-deletion as query against temperature-sensitive collection, see Table S12 for ts allele (hit)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDC48 SGS1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2536BioGRID
363563
SGS1 CDC48
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2839BioGRID
2062773
CDC48 SGS1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.244BioGRID
1965806
SGS1 CDC48
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

High-BioGRID
2340613
SGS1 CDC48
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
2898323
SGS1 CDC48
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

High0.0156BioGRID
822754

Curated By

  • BioGRID