A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.

Ohkura H, Kinoshita N, Miyatani S, Toda T, Yanagida M

S. pombe dis mutants block mitotic chromosome disjunction in a manner reminiscent of aneuploidy formation, and belong to three distinct genes, dis1-dis3. We cloned two independent genomic DNAs that complemented both the cold-sensitive and caffeine-hypersensitive phenotype of dis2-11. These genes, dis2+ and a suppressor sds21+, encode proteins (calculated MW 37,000) with similar predicted amino acid sequences. dis2+ and sds21+ have ... [more]

Cell Jun. 16, 1989; 57(6);997-1007 [Pubmed: 2544298]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DIS2 SDS21	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Alvarez-Tabares I (2007)	Low	-	BioGRID	-
DIS2 SDS21	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Kinoshita N (1990)	Low	-	BioGRID	247622

Curated By

BioGRID

Interaction Summary

DIS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphoprotein phosphatase activity [IDA]protein serine/threonine phosphatase activity [IDA]

Gene Ontology Cellular Component

SDS21