WNK1
Gene Ontology Biological Process
Gene Ontology Molecular Function
WNK3
Gene Ontology Biological Process
- intracellular signal transduction [ISS]
- negative regulation of apoptotic process [IMP]
- positive regulation of calcium ion transport [IMP]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- positive regulation of ion transmembrane transporter activity [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of rubidium ion transmembrane transporter activity [IDA]
- positive regulation of rubidium ion transport [IDA]
- positive regulation of sodium ion transmembrane transporter activity [IDA, IMP]
- positive regulation of sodium ion transport [IDA]
- protein autophosphorylation [IDA]
- protein phosphorylation [ISS]
- regulation of ion homeostasis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WNK3 WNK1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 20536 | BioGRID | 3486408 | |
| WNK1 WNK3 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3677399 | |
| WNK1 WNK3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.0342 | BioGRID | 3584538 |
Curated By
- BioGRID