BAIT

GCN2

AAS1, AAS102, NDR2, serine/threonine-protein kinase GCN2, L000000681, YDR283C
Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
Saccharomyces cerevisiae (S288c)
PREY

TIF5

SUI5, translation initiation factor eIF5, L000002305, YPR041W
Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation.

Singh CR, Lee B, Udagawa T, Mohammad-Qureshi SS, Yamamoto Y, Pavitt GD, Asano K

In eukaryotic translation initiation, the eIF2.GTP/Met-tRNA(i)(Met) ternary complex (TC) binds the eIF3/eIF1/eIF5 complex to form the multifactor complex (MFC), whereas eIF2.GDP binds the pentameric factor eIF2B for guanine nucleotide exchange. eIF5 and the eIF2Bvarepsilon catalytic subunit possess a conserved eIF2-binding site. Nearly half of cellular eIF2 forms a complex with eIF5 lacking Met-tRNA(i)(Met), and here we investigate its physiological significance. ... [more]

EMBO J. Oct. 04, 2006; 25(19);4537-46 [Pubmed: 16990799]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • genetic complex
  • overexpression of TIF5 enhances the Gcd- phenotype of a gcn2/gcd6 double mutant allowing robust growth on 3-AT

Curated By

  • BioGRID