BAIT

CDC16

anaphase promoting complex subunit CDC16, L000000256, YKL022C
Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

MPS1

PAC8, RPK1, serine/threonine/tyrosine protein kinase MPS1, L000001698, YDL028C
Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase.

Liu C, van Dyk D, Choe V, Yan J, Majumder S, Costanzo M, Bao X, Boone C, Huo K, Winey M, Fisk H, Andrews B, Rao H

Ufd2 is a U-box containing ubiquitylation enzyme that promotes ubiquitin chain assembly on substrates. The physiological function of Ufd2 remains poorly understood. Here we show that ubiquitylation and degradation of the cell cycle kinase Mps1, a known target of APC E3, requires Ufd2 enzyme. Yeast cells lacking UFD2 exhibit altered chromosome stability and several spindle related phenotypes, expanding the ... [more]

Unknown Nov. 01, 2011; 0(0); [Pubmed: 22045814]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • growth of cdc16-1 is impaired by Mps1 overexpression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDC16 MPS1
Dosage Growth Defect
Dosage Growth Defect

A genetic interaction is inferred when over expression or increased dosage of one gene causes a growth defect in a strain that is mutated or deleted for another gene.

Low-BioGRID
429322
CDC16 MPS1
Dosage Lethality
Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Low-BioGRID
153868

Curated By

  • BioGRID