PRKAA1
Gene Ontology Biological Process
- activation of MAPK activity [NAS]
- cell cycle arrest [TAS]
- cellular response to glucose starvation [ISS]
- cellular response to nutrient levels [ISS]
- fatty acid homeostasis [ISS]
- glucose homeostasis [ISS]
- insulin receptor signaling pathway [TAS]
- lipid biosynthetic process [ISS]
- negative regulation of TOR signaling [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of glucosylceramide biosynthetic process [NAS]
- negative regulation of lipid catabolic process [ISS]
- positive regulation of autophagy [ISS]
- positive regulation of cholesterol biosynthetic process [NAS]
- positive regulation of gene expression [IDA]
- positive regulation of glycolytic process [ISS]
- protein phosphorylation [IDA]
- regulation of circadian rhythm [ISS]
- regulation of energy homeostasis [ISS]
- response to gamma radiation [ISS]
- response to hypoxia [NAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRKAB2
Gene Ontology Biological Process
- carnitine shuttle [TAS]
- cell cycle arrest [TAS]
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- insulin receptor signaling pathway [TAS]
- membrane organization [TAS]
- protein phosphorylation [IDA]
- regulation of fatty acid biosynthetic process [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]
Additional Notes
- exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 446403 | |
| PRKAB2 PRKAA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 446448 | |
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 2448 | BioGRID | 3483659 | |
| PRKAB2 PRKAA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 5848 | BioGRID | 3480854 | |
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| PRKAB2 PRKAA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3363963 | |
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9977 | BioGRID | 3550320 | |
| PRKAB2 PRKAA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2219226 | |
| PRKAB2 PRKAA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3139519 | |
| PRKAA1 PRKAB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| PRKAB2 PRKAA1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.9553 | BioGRID | 1267388 | |
| PRKAB2 PRKAA1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| PRKAA1 PRKAB2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| PRKAA1 PRKAB2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID